Impact of Tralopyril and Triazolyl Glycosylated Chalcone in Human Retinal Cells' Lipidome

• Type: Article in International Scientific Journal
• Date: 2022

Authors

Vilas Boas, C (Author) Other; Running, L (Author) Other; Pereira, D (Author) Other; Honorina Cidade (Author) FFUP; Marta Correia da Silva (Author) FFUP; Atilla Gokcumen, GE (Author) Other; Aga, DS (Author) Other

Journal

Title: Molecules

Vol. 126

Final page: 5247

ISSN: 1420-3049

Publisher: MDPI

Indexing

ISI Web of Knowledge - 5 Citations

Scopus - 5 Citations

Other information

Authenticus ID: P-00X-40S

DOI: 10.3390/molecules27165247

Abstract (EN): Antifouling (AF) coatings containing booster biocides are used worldwide as one of the most cost-effective ways to prevent the attachment of marine organisms to submerged structures. Nevertheless, many of the commercial biocides, such as Econea((R)) (tralopyril), are toxic in marine environments. For that reason, it is of extreme importance that new efficient AF compounds that do not cause any harm to non-target organisms and humans are designed. In this study, we measured the half-maximal inhibitory concentration (IC50) of a promising nature-inspired AF compound, a triazolyl glycosylated chalcone (compound 1), in an immortalized human retinal pigment epithelial cell line (hTERT-RPE-1) and compared the results with the commercial biocide Econea((R)). We also investigated the effects of these biocides on the cellular lipidome following an acute (24 h) exposure using liquid chromatography quadrupole time-of-flight mass spectrometry (LC-Q-TOF/MS). Our results showed that compound 1 did not affect viability in hTERT-RPE-1 cells at low concentrations (1 mu M), in contrast to Econea((R)), which caused a 40% reduction in cell viability. In total, 71 lipids were found to be regulated upon exposure to 10 mu M of both compounds. Interestingly, both compounds induced changes in lipids involved in cell death, membrane modeling, lipid storage, and oxidative stress, but often in opposing directions. In general, Econea((R)) exposure was associated with an increase in lipid concentrations, while compound 1 exposure resulted in lipid depletion. Our study showed that exposure to human cells at sublethal Econea((R)) concentrations results in the modulation of several lipids that are linked to cell death and survival.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 11