Gallic acid markedly stimulates GLUT1-mediated glucose uptake by the AsPC-1 pancreatic cancer cell line

• Type: Article in International Scientific Journal
• Date: 2023

Authors

Torres, SM (Author) Other; Carmo, FP (Author) Other; Monteiro, LC (Author) Other; Silva, C (Author) FMUP; Andrade, N (Author) Other; Martel, F (Author) FMUP

Journal

Title: Canadian Journal of Physiology and Pharmacology

Vol. 101

Pages: 90-105

ISSN: 0008-4212

Publisher: NRC Research Press

Indexing

ISI Web of Knowledge - 0 Citations

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Other information

Authenticus ID: P-00X-W6A

DOI: 10.1139/cjpp-2022-0260

Abstract (EN): Phenolic acids are recognized as chemopreventive and chemotherapeutic agents. Altered glucose and glutamine metabolism are recognized hallmarks of cancer cells. We aimed to test the influence of phenolic acids on glucose and glutamine cellular uptake by a breast (MCF-7) and a pancreatic (AsPC-1) cancer cell line. Several phenolic acids (caffeic, ferrulic, proctocatechuic, coumaric and gallic acid) affected 3H-glutamine and/or 3H-deoxy-D-glucose (3H-DG) uptake. Gallic acid (100 mu M) caused a 3-fold increase in 3H-DG uptake by AsPC-1 cells, associated with a 3.7-fold increase in lactic acid production. Gallic acid stimulated GLUT1-mediated 3H-DG uptake and increased the affinity of this transporter for 3H-DG. We further verified that gallic acid does not change GLUT1 transcription rates and cellular redox state and that its effect does not involve PI3K, mTOR and MAP kinases and is not associated with a proproliferative effect. Gallic acid also increased 3H-DG uptake by MCF-7 cells, although less potently. Further investigation is necessary to elucidate the cellular pathways involved in this effect of gallic acid.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 16