Adhesion of human leukocytes on mixtures of hydroxyl- and methyl-terminated self-assembled monolayers: Effect of blood protein adsorption

• Type: Article in International Scientific Journal
• Date: 2010

Authors

Judite N Barbosa (Author) ICBAS; Martins, MCL (Author) Other; Sidonio C Freitas (Author) Other; Ines C Goncalves (Author) FEUP; Artur P Aguas (Author) ICBAS; Mario A Barbosa (Author) FEUP

Journal

Title: Journal of Biomedical Materials Research - Part A

Vol. 93A No. 1

Pages: 12-19

ISSN: 1549-3296

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 9 Citations

Scopus - 13 Citations

Scientific classification

FOS: Engineering and technology > Environmental biotechnology

CORDIS: Technological sciences > Engineering > Biomaterial engineering

Other information

Authenticus ID: P-003-7W5

DOI: 10.1002/jbm.a.32480

Abstract (EN): The adhesion of human leukocytes to nanostructured surfaces with different chemical properties and the effect of protein adsorption were investigated. Self-assembled monolayers (SAMs) prepared with mixtures of methyl- and hydroxyl-terminated alkanethiols in different Percentages oil gold were used. The surfaces were pre-immersed in distinct protein solutions (human serum albumin, human fibrinogen, and autologous plasma). Adherent leukocytes were analyzed both by light and SEM. SAMs submitted to pre-immersion in plasma presented higher numbers of adherent leukocytes in the pure OH-terminated SAM, whereas methyl-terminated Surfaces accounted for the lowest number of adherent cells. We observed a general increase ill the number of adherent human leukocytes as the percentage of OH groups on the Surface of the SAMs increased for all the pre-immersion conditions investigated. The number of adherent human leukocytes is highly influenced by the pre-immersion conditions used, and this observation is particularly relevant in the case of the methyl-terminated SAMs. The results obtained demonstrate that surface chemistry has a major influence in leukocyte adhesion to biomaterials, and that pre-immersion in protein solutions has a determinant effect in leukocyte adhesion. (C) 2009 Wiley Periodicals, Inc. J Biomed Mater Res 93A: 12-19, 2010

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8