In vitro models of periodontal cells: a comparative study of long-term gingival, periodontal ligament and alveolar bone cell cultures in the presence of beta-glycerophosphate and dexamethasone

• Type: Article in International Scientific Journal
• Date: 2007

Authors

Trigo Cabral, MCT (Author) Other; Costa, MA (Author) ICBAS; Fernandes MH (Author) FMDUP

Journal

Title: Journal of Materials Science: Materials in Medicine

Vol. 18 No. 12

Pages: 1079-1088

ISSN: 0957-4530

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 17 Citations

Scopus - 20 Citations

Other information

Authenticus ID: P-004-9TQ

DOI: 10.1007/s10856-007-0134-1

Abstract (EN): Human gingival (HG), periodontal ligament (HPL) and alveolar bone (HAB) cells (first subculture) were cultured (10(4) cells/cm(2)) for 35 days in alpha-Minimal Essential Medium supplemented with 10% fetal bovine serum in the presence of (i) ascorbic acid (AA, 50 mu g/mL), (ii) AA + beta-glycerophosphate (beta GP, 10 mM) and (iii) AA + beta GP + dexamethasone (Dex, 10 nM). Cultures were assessed for cell attachment and spreading, cell proliferation, alkaline phosphatase (ALP) and acid phosphatase (ACP) activities and matrix mineralization. HG cell cultures presented a high proliferation rate, a low ability to synthesize ALP and ACP and the formation of a non-mineralized extracellular matrix, regardless the experimental situation. HPL cell cultures were very sensitive to the culture conditions and showed a high proliferation rate, synthesis of moderate levels of ALP and ACP and a modest matrix mineralization in the presence of AA + beta GP + Dex. HAB cell cultures presented a growth rate lower than that of HG and HPL cells, a high ALP activity and comparatively low levels of ACP, and the ready formation of a heavy mineralized matrix in the presence of beta GP. In the three periodontal cell cultures, Dex enhanced cell proliferation and expression of osteoblastic markers. Results showed that beta GP and Dex allowed the modulation of the cell proliferation/differentiation behavior within the proposed physiological and regenerative capabilities of these periodontal cells.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10