Effect of cryoprotectants on the reconstitution of silica nanoparticles produced by sol-gel technology

• Type: Article in International Scientific Journal
• Date: 2015

Authors

Andreani, T (Author) Other; Kiill, CP (Author) Other; de Souza, ALR (Author) Other; Fangueiro, JF (Author) Other; Doktorovová, S (Author) Other; Garcia, ML (Author) Other; Gramiao, MPD (Author) Other; Silva, AM (Author) Other; Souto, EB (Author) Other

Journal

Title: Journal of Thermal Analysis and Calorimetry

Vol. 120

Pages: 1001-1007

ISSN: 1388-6150

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 13 Citations

Scopus - 14 Citations

Other information

Authenticus ID: P-00A-BMJ

DOI: 10.1007/s10973-014-4275-4

Abstract (EN): Freeze-drying has widely been applied to improve the stabilization of colloidal drug carriers. In the present study, the effect of cryoprotectants on the physicochemical characteristics of silica nanoparticles (SiNP) during the freeze-drying process has been extensively investigated. SiNP were synthesized by sol-gel technology and freeze-dried in the presence of sorbitol, trehalose, and/or mannitol at different concentrations and ratios. Dynamic light scattering (DLS), atomic force microscopy (AFM), X-ray diffraction analysis (XRD), and differential scanning calorimetry (DSC) have been used for particle characterization after freeze-drying. Based on the obtained results, SiNP in the presence of mannitol showed a more crystalline behavior in comparison to nanoparticles with sorbitol or trehalose (confirmed by DSC and XRD). SiNP in the presence of trehalose showed a more crystalline structure than SiNP in the presence of sorbitol. However, trehalose was more efficient in preserving the particle size of nanoparticles during the freeze-drying process. The optimal concentration of trehalose for preserving silica nanoparticles was 10 % at a ratio of (1:1). During the freeze-drying process, trehalose is able to replace water molecules due to the strong interaction via hydrogen bounds between silanol groups present in SiNP surface and the sugar, forming a stable layer around the particle and thus preserving the particle physical properties.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7