Antiaggregant and toxic properties of different solutions on hemocytes of three freshwater bivalves

• Type: Article in International Scientific Journal
• Date: 2013

Authors

Hinzmann, MF (Author) Other; Lopes Lima, M (Author) Other; goncalves, j (Author) ICBAS; machado, j (Author) ICBAS

Journal

Title: Toxicological and Environmental Chemistry

Vol. 95

Pages: 790-805

ISSN: 0277-2248

Publisher: Taylor & Francis

Indexing

ISI Web of Knowledge - 9 Citations

Scopus - 13 Citations

Other information

Authenticus ID: P-006-B9F

DOI: 10.1080/02772248.2013.818149

Abstract (EN): Hemocytes are main blood components of bivalves playing important roles in toxicological and immune responses. Consequently, a study on these cells may be useful to understand the invertebrate immunological systems. The aim of the present study was to find the best antiaggregant solution, based on its capacity of preserving the natural morphology and viability of hemocytes from the freshwater bivalves, Anodonta cygnea, Unio delphinus, and Corbicula fluminea. Hemocytes from the three species were collected and maintained in different antiaggregant solutions, i.e., ethylenediaminetetraacetic acid (EDTA), EDTA-citrate, modified Alsever solution (MAS), N-ethylmaleimide (NEM), NEM-NaCl, and heparin. The cell morphology and viability were analyzed periodically with light microscopy techniques. Between these assays with various antiaggregant solutions, the best results were obtained with NEM at a concentration of 0.05 mol center dot L-1. It is also shown that, as expected, the osmolarity of the antiaggregant solutions plays a very important role in cell viability. Based on this study, NEM at 0.05 mol center dot L-1 was considered an adequate antiaggregant solution for future research on immunological and toxicological responses and other physiological studies of freshwater bivalve hemocytes.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 16