A protocol for micropropagation of the medicinal species Tuberaria lignosa provides ploidy true-to-type plants with high antioxidant capacity

• Type: Article in International Scientific Journal
• Date: 2022

Authors

Rebelo, D (Author) Other; Mariz Ponte, N (Author) Other; Loureiro, J (Author) Other; Castro, S (Author) Other; Debiasi, C (Author) Other; Domingues, J (Author) Other; Santos, C (Author) FCUP; Goncalves, JC (Author) Other

Journal

Title: Plant Cell, Tissue and Organ Culture

Vol. 150

Pages: 599-609

ISSN: 0167-6857

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 2 Citations

Scopus - 3 Citations

Other information

Authenticus ID: P-00W-MVN

DOI: 10.1007/s11240-022-02313-5

Abstract (EN): Tuberaria lignosa (Cistaceae family) has excellent properties as an antioxidant, anti-inflammatory, antimicrobial, and antiproliferative/anti-tumoural. This work aimed to establish a routine micropropagation protocol of T. lignosa to supply high-quality plants for the pharmaceutical industry. Apical shoot cuttings (0.5 cm long) were grown on basal medium Murashige and Skoog (MS) and 1/2MS for 60 days with different growth regulators. 1/2MS supplemented with 1 mg L-1 benzylaminopurine (BAP) provided the best results with shoots showing better growth rates and no vitrification or browning. After 60 days, the best results for shoot proliferation were achieved on 1/2MS with 1 mg L-1 mT, with 100% explants responding and similar to 4 new shoots formed per explant. For elongation and rooting, after 30 days, 1 mg L-1 indoleacetic acid (IAA) provided 100% of rooting (similar to 18 roots per shoot), and resulting plants were vigorous, also with no signs of vitrification or browning. For acclimatization, the substrate Jiffy Plug provided the best results after 60 days, with a survival rate of 68.42%. In the subsequent 30 days in greenhouse conditions, all plants survived. Acclimatized plants had still less proteins, but high activity of antioxidant enzymes compared with the mother plants, supporting that these cloned plants may be of interest to the pharmaceutical industry. No changes in the DNA-ploidy were detected between micropropagated and the mother plants. The protocol described here provides, after 8 months of explant establishment, a high number of plants/explants, revealing a high potential for future use in this species and in other medicinal and economically relevant species, and in species in need of ex-situ conservation measures.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 11