Dual pulsed laser deposition of Ag nanoparticles on calcium phosphate coatings for biomedical applications

• Type: Article in International Scientific Journal
• Date: 2022

Authors

Lopez-Alvarez, M (Author) Other; Gonzalez-Rodriguez, L (Author) Other; Gontad, F (Author) Other; Teixeira-Santos, R (Author) FEUP; Doiro, M (Author) Other; Alvarez-Gomez, L (Author) Other; Filipe Mergulhão (Author) FEUP; Gonzalez, P (Author) Other; Serra, J (Author) Other

Journal

The Journal is awaiting validation by the Administrative Services.

Title: BIOMEDICAL PHYSICS & ENGINEERING EXPRESS

Vol. 8

ISSN: 2057-1976

Indexing

ISI Web of Knowledge - 0 Citations

Scopus

Other information

Authenticus ID: P-00X-ABN

DOI: 10.1088/2057-1976/ac9846

Abstract (EN): Pulsed laser deposition (PLD) represents a promising bottom-up methodology for the synthesis and transference of nanoparticles to the surface of a biomedical device. Silver (Ag) nanoparticles directly incorporated on the metallic implant emerge as an alternative strategy for local action against prosthetic joint-associated infections. In the present research, a dual sequential PLD process is proposed to obtain a bilayer coating with (1) a bio-derived calcium phosphate (CaP) layer, to provide osteointegrative properties and (2) the controlled growth of the Ag nanoparticles over it, ranging the number of laser pulses from 100 to 500. The characterization by SEM, EDS, TEM, XPS and AFM revealed the uniform deposit of Ag rounded nanoparticles, with a narrow mean size distribution, in the original non-oxidized metallic state. Moreover, given the evidences from XPS and AFM techniques, the occurrence of a coalescence phenomenon from 400 pulses onwards was proposed together with the expected positive linear relation between the number of pulses and Ag contribution with a deposition rate of 0.05 at. % of Ag per pulse. Conversely, the decrease in roughness as the Ag content increased was also verified. Finally, the expected bacteriostatic activity for these PLD deposited metallic state Ag nanoparticles against the bacterial strain Staphylococcus aureus was confirmed. Moreover, the evaluation of the osteoblast-like MG-63 cells viability on the Ag(100-500)-CaP coatings revealed a significant increased proliferation (p > 0.05) on the Ag100-CaP coating compared to the control (AgO-CaP). When same coating was evaluated against S. aureus the effect was not significant. The possibility of modulating the amount of nanoparticles in the bilayer coating to obtain a greater or lesser effect in combination with CaP was revealed.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 12