A NOS1 gene polymorphism associated with asthma and specific immunoglobulin e response to mite allergens in a Colombian population

• Type: Article in International Scientific Journal
• Date: 2007

Authors

Martinez, B (Author) Other; Barrios, K (Author) Other; Vergara, C (Author) Other; Mercado, D (Author) Other; Jimenez, S (Author) Other; Gusmao, L (Author) Other; Caraballo, L (Author) Other

Journal

Title: International Archives of Allergy and Immunology

Vol. 144

Pages: 105-113

ISSN: 1018-2438

Publisher: Karger

Indexing

ISI Web of Knowledge - 22 Citations

Scopus - 25 Citations

Other information

Authenticus ID: P-004-DGR

DOI: 10.1159/000103221

Abstract (EN): Background: Nitric oxide (NO) is involved in asthma pathogenesis and is synthesized by three isoforms of NO synthase, one of them encoded by NOS1 gene. The CA-repeat and the C5266T SNP in NOS1 exon 29 have been associated with asthma and IgE levels. We thought to test the association of asthma and asthma-related phenotypes with the exon 29 CA-repeat and the C5266T SNP in a Colombian population sample. Methods: The CA-repeat and the C5266T SNP were genotyped in 167 asthmatics and 166 controls using PCR-based fragment length polymorphism and TaqMan assay. We also determined total and mite-specific IgE against Blomia tropicalis and Dermatophagoides pteronyssinus Results: Three new CA-repeat alleles ,14, 23 and 24 repeats were detected. Allele comprising 16 repeats was associated with asthma (OR: 1.90 (CI 1.22 - 2.97, p(c) = 0.028) and low total (p(c) = 0.02) and specific IgE to B. tropicalis (p(c) < 0.0001) and D. pteronyssinus (p(c) < 0.0001). We found no association of the C5266T SNP and asthma or IgE levels. Conclusion: NOS1 exon 29 CA-repeat may be a risk factor for asthma susceptibility and mite specific IgE response in a Colombian population. Copyright (c) 2007 S. Karger AG, Basel.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9