Augmin-dependent microtubule self-organization drives kinetochore fiber maturation in mammals

• Type: Article in International Scientific Journal
• Date: 2022

Authors

Almeida, AC (Author) Other; Soares-de-Oliveira, J (Author) Other; Drpic, D (Author) Other; Cheeseman, LP (Author) Other; Damas, J (Author) Other; Lewin, HA (Author) Other; Larkin, DM (Author) Other; Paulo Aguiar (Author) FMUP; Pereira, AJ (Author) Other; Helder Maiato (Author) FMUP

Journal

Title: Cell Reports

Vol. 39

ISSN: 2211-1247

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 4 Citations

Scopus - 5 Citations

Other information

Authenticus ID: P-00W-CJH

DOI: 10.1016/j.celrep.2022.110610

Abstract (EN): Chromosome segregation in mammals relies on the maturation of a thick bundle of kinetochore-attached microtubules known as k-fiber. How k-fibers mature from initial kinetochore microtubule attachments remains a fundamental question. By combining molecular perturbations and phenotypic analyses in Indian muntjac fibroblasts containing the lowest known diploid chromosome number in mammals (2N = 6) and distinctively large kinetochores, with fixed/live-cell super-resolution coherent-hybrid stimulated emission depletion (CH-STED) nanoscopy and laser microsurgery, we demonstrate a key role for augmin in kinetochore micro tubule self-organization and maturation, regardless of pioneer centrosomal microtubules. In doing so, augmin promotes kinetochore and interpolar microtubule turnover and poleward flux. Tracking of microtubule growth events within individual k-fibers reveals a wide angular dispersion, consistent with augmin-mediated branched microtubule nucleation. Augmin depletion reduces the frequency of kinetochore microtubule growth events and hampers efficient repair after acute k-fiber injury by laser microsurgery. Together, these findings underscore the contribution of augmin-mediated microtubule amplification for k-fiber self -organization and maturation in mammals.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 27