Relative hydrophobicity of (PEG or Ucon)-salt ATPSs

• Type: Article in International Conference Proceedings Book
• Date: 2008

Authors

Sara C. Silvério (Author) Other; Pedro P. Madeira (Author) Other; Oscar Rodriguez (Author) Other; José A. Teixeira (Author) Other; Eugénia A. Macedo (Author) FEUP

Conference proceedings International

Title: Book of Abstracts of the 10th International Chemical Conference and Biological Engineering Conference - CHEMPOR 2008

Pages: 2168-2171

CHEMPOR 2008 - 10th International Chemical Conference and Biological Engineering Conference

Braga, Portugal, 4 a 6 de Setembro de 2008

Indexing

ISI Proceedings

Scientific classification

FOS: Engineering and technology > Other engineering and technologies

Other information

Abstract (EN): Aqueous Two-Phase Systems (ATPSs) are biphasic systems composed mainly by water. ATPSs are obtained upon mixing of two aqueous solutions of certain polymers or a polymer and a salt (above certain critical conditions, e.g. concentration, temperature). These systems are commonly indicated for the extraction of biomolecules. In this work, the partition coefficients for a series of five dinitrophenylated amino-acids (ranging from glycine to amino-caprylic acid) were determined experimentally in five different polymer-salt ATPSs (polymers: PEG or Ucon; salts: Na2SO4, Li2SO4 or (NH4)2SO4) at 23ºC. Values of the free energy of transfer of a methylene group, ¿G(CH2), for the five ATPSs were obtained from the partition coefficients and compared with ¿G(CH2) previously obtained for PEG-Na2SO4 (Rodríguez et al., 2007). Ucon-salt ATPSs presented higher values of ¿G(CH2) than the corresponding PEG-salt systems, witch indicates that the Ucon-rich phase is more hydrophobic than the PEG-rich phase.

Language: English

Type (Professor's evaluation): Scientific

Notes: ISBN: 978-972-97810-4-9

No. of pages: 4