Significance of trisomy 7 and 12 in thyroid lesions with follicular differentiation: A cytogenetic and in situ hybridization study

• Type: Article in International Scientific Journal
• Date: 1999

Authors

Rogue, L (Author) Other; Serpa, A (Author) Other; Clode, A (Author) Other; CASTEDO, S (Author) FMUP; Soares, J (Author) Other

Journal

Title: Laboratory Investigation

Vol. 79

Pages: 369-378

ISSN: 0023-6837

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 14 Citations

Scopus - 21 Citations

Other information

Authenticus ID: P-001-4RA

Abstract (EN): In a group of benign and malignant follicular thyroid lesions, previously analyzed by conventional cytogenetics, single- and double-target fluorescence in situ hybridization studies with pericentromeric probes specific for chromosomes 7 and 12 were performed, by using isolated nuclei from paraffin-embedded specimens of: 11 goiters, 21 adenomas, 9 follicular carcinomas, and adjacent normal thyroid tissue. Nonisotopic in situ hybridization with the same probes was used in 4-mu m histologic sections of tour follicular carcinomas. By fluorescence in situ hybridization analysis, the percentage of goiters, adenomas, and follicular carcinomas with gains of No. 7 was 18.2%, 52.4%, and 66.0%, respectively, and with gains of No. 12 was 9.0%, 42.9%, and 66.0%, respectively. The percentage of the same lesions (goiters, adenomas, carcinomas) exhibiting polysomies of No. 7 and No. 12, as assessed by cytogenetic analysis, was 5.0% and 0.0%, 20.0% and 20.0%, and 15.8% and 10.5%, respectively. Numerical alterations of these chromosomes were not observed in normal tissue. These findings reveal that gain of chromosomes 7 and 12 is a characteristic of the morphologically altered thyroid tissue; polysomies of chromosomes 7 and 12 are more frequent in thyroid lesions than it can be detected by conventional cytogenetic studies; the increasing frequency of polysomies of chromosomes 7 and 12 from hyperplastic lesions to benign and malignant tumors seem to substantiate the existence of a multistep pathway. ie, normal thyroid --> goiter --> adenoma --> follicular carcinoma in the pathogenesis of some thyroid neoplasms.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10