NUCLEOLAR ALTERATIONS INDUCED BY 4-AMINOPYRAZOLO(3,4-D)PYRIMIDINE IN ADRENAL-CORTEX AND LIVER-CELLS OF RAT

• Type: Article in International Scientific Journal
• Date: 1987

Authors

Almeida, Henrique (Author) FMUP; MAGALHAES, M (Author) Other; MAGALHAES, MC (Author) Other

Journal

Title: Cell and Tissue Research

Vol. 248

Pages: 231-234

ISSN: 0302-766X

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 3 Citations

Scopus - 2 Citations

Other information

Authenticus ID: P-001-VCQ

DOI: 10.1007/bf01239986

Abstract (EN): The nucleolar ultrastructural changes produced in adrenal zona fasciculata cells and hepatocytes of rats by 4-aminopyrazolo(3,4-d) pyrimidine (4-APP), an inhibitor of the lipoprotein synthesis, are described. Male rats were injected intraperitoneally for three consecutive days with 4-APP in sodium phosphate buffer (50 mg/Kg/day). On the 4th day, the animals were sacrificed and their adrenals and liver were processed for EM. The nucleoli of the adrenal zona fasciculata cells showed nucleolar fragmentation with loss of their normal reticular appearance, separation of fibrillar and granular components, extensive vacuolization and evidence of fibrillar centers. The hepatocyte nucleoli also exhibited fragmentation. These changes seem to be the morphological counterpart of alterations in rRNA synthesis and processing, since 4-APP inhibits "de novo" purine synthesis and thus interferes with its incorporation into RNA and DNA. © 1987 Springer-Verlag.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 4