beta(2) Adrenoceptors are underexpressed in peripheral blood mononuclear cells and associated with a better metabolic profile in central obesity

• Type: Article in International Scientific Journal
• Date: 2017

Authors

Leite, F (Author) Other; Lima, M (Author) Other; Marino, F (Author) Other; Cosentino, M (Author) Other; ribeiro, l (Author) FMUP

Journal

Title: International Journal of Medical Sciences

Vol. 14

Pages: 853-861

ISSN: 1449-1907

Publisher: Ivyspring International Publisher

Indexing

ISI Web of Knowledge - 7 Citations

Scopus - 7 Citations

Other information

Authenticus ID: P-00M-XHS

DOI: 10.7150/ijms.19638

Abstract (EN): Background: Central obesity (CO) is an inflammatory disease. Because immune cells and adipocytes are catecholamines(CA)-producing cells, we studied the expression of adrenoceptors (AR) in peripheral blood mononuclear cells (PBMCs) hypothesizing a distinct adrenergic pattern in inflammatory obesity. Methods: AR expression was assessed in blood donors categorized by waist circumference (WC) (CO: WC >= 0.80 m in women and >= 0.94 m in men). Following a pilot study for all AR subtypes, we measured beta(2)AR expression in fifty-seven individuals and correlated this result with anthropometric, metabolic and inflammatory parameters. A ratio (R) between AR mRNA of CO and non-CO<0.5 was considered under and >2.0 over expression. Results: The pilot study revealed no differences between groups, except for beta(2)AR mRNA. CO individuals showed underexpression of beta(2)AR relatively to those without CO (R=0.08; p=0.009). beta(2)AR expression inversely correlated with triacylglycerol (r=-0.271; p=0.041), very low-density lipoprotein-cholesterol (r=-0.313; p=0.018) and leptin (r=-0.392; p=0.012) and positively with high-density lipoprotein-cholesterol (r=0.310: p=0.045) plasma levels. Multiple logistic regression analysis showed a protective effect of beta(2)AR expression (>= 2x10-6) [odds ratio (OR) 0.177 with respective confidence interval of 95% (95% CI) (0.040-0.796)] for the occurrence of CO. A higher association was found for women as compared to men ( 9: 1) [OR 8.972 (95% CI) (1.679-47.949)]. Conclusion: PBMCs beta(2)AR, underexpressed in centrally obese, are associated with a better metabolic profile and showed a protective role for the development of CO. The discovery of beta(2)AR as a new molecular marker of obesity subphenotypes in PBMCs might contribute to clarify the adrenergic immunomodulation of inflammatory obesity.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9