Reliable blood cancer cells' telomere length evaluation by qPCR

• Type: Article in International Scientific Journal
• Date: 2020

Authors

Ropio, J (Author) Other; Chebly, A (Author) Other; Ferrer, J (Author) Other; Prochazkova Carlotti, M (Author) Other; Idrissi, Y (Author) Other; Azzi Martin, L (Author) Other; Cappellen, D (Author) Other; Pham Ledard, A (Author) Other; Soares, P (Author) FMUP; Merlio, JP (Author) Other; Chevret, E (Author) Other

Journal

The Journal is awaiting validation by the Administrative Services.

Title: CANCER MEDICINE

Vol. 9

Pages: 3153-3162

ISSN: 2045-7634

Indexing

ISI Web of Knowledge - 5 Citations

Scopus - 5 Citations

Other information

Authenticus ID: P-00R-VTH

DOI: 10.1002/cam4.2816

Abstract (EN): Background Telomere shortening is linked to a range of different human diseases, hence reliable measurement methods are needed to uncover such associations. Among the plethora of telomere length measurement methods, qPCR is reported as easy to conduct and a cost-effective approach to study samples with low DNA amounts. Methods Cancer cells' telomere length was evaluated by relative and absolute qPCR methods. Results Robust and reproducible telomere length measurements were optimized taking into account a careful reference gene selection and by knowing the cancer cells ploidy. qPCR data were compared to "gold standard" measurement from terminal restriction fragment (TRF). Conclusions Our study provides guidance and recommendations for accurate telomere length measurement by qPCR in cancer cells, taking advantage of our expertise in telomere homeostasis investigation in primary cutaneous T-cell lymphomas. Furthermore, our data emphasize the requirement of samples with both, high DNA quality and high tumor cells representation.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10