Cellulose phosphates as biomaterials. In vivo biocompatibility studies

• Type: Article in International Scientific Journal
• Date: 2002

Authors

J. C. Fricain (Author) Other; Pedro Lopes Granja (Author) Other; Mário Adolfo Barbosa (Author) FEUP; B. de Jéso (Author) Other; N. Barthe (Author) Other; C. Baquey (Author) Other

Journal

Title: Biomaterials

Vol. 23 No. 4

Pages: 971-980

ISSN: 0142-9612

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 65 Citations

ISI Web of Science

Scopus - 73 Citations

COMPENDEX

Scientific classification

CORDIS: Technological sciences > Engineering > Biomaterial engineering ; Technological sciences > Engineering > Biomedical enginnering

FOS: Engineering and technology > Environmental biotechnology

Other information

Authenticus ID: P-000-QBT

DOI: 10.1016/s0142-9612(01)00152-1

Abstract (EN): Femoral implantation of regenerated cellulose hydrogels revealed their biocompatibility, but a complete osseointegration could not be observed. Phosphorylation was therefore envisaged as the means to enhance cellulose bioactivity. In vitro studies showed that regenerated cellulose hydrogels promote bone cells attachment and proliferation but do not mineralize in acellular simulated physiological conditions. On the contrary, phosphorylated cellulose has shown an opposite behavior, by inducing the formation of a calcium phosphate layer in simulated physiological conditions, but behaving as a poor substrate for bone cells attachment and proliferation. In order to investigate the in vivo behavior of these materials, and assess the influence of mineralization induction ability vs. bone cells compatibility, unmodified and phosphorylated cellulose hydrogels were implanted in rabbits for a maximum period of 6 months and bone regeneration was investigated. Despite the difficulties arising from the retraction of cellulose hydrogels upon dehydration during the preparation of retrieved implants, histological observations showed no inflammatory response after implantation, with bone intra-spongious regeneration of cells and the integration of the unmodified as well as the phosphorylated cellulose implants. After a maximum implantation period of 6 months, histological observations, histomorphometry and the measurement of the amount of Ca-45 incorporated in the surrounding tissue indicated a slightly better osseointegration of phosphorylated cellulose, although no significant differences between the two materials were found.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10