Bioactive peptides in ovine and caprine cheeselike systems prepared with proteases from Cynara cardunculus

• Type: Article in International Scientific Journal
• Date: 2006

Authors

Silva, SV (Author) Other; Pihlanto, A (Author) Other; F. Xavier Malcata (Author) Other

Journal

Title: Journal of Dairy Science

Vol. 89 No. 7

Pages: 3336-3344

ISSN: 0022-0302

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 62 Citations

Scopus - 82 Citations

Other information

Authenticus ID: P-004-HKF

Abstract (EN): The potential angiotensin-converting enzyme (ACE) inhibitory and antioxidant activities of peptides in water-soluble extracts, obtained from raw and sterilized ovine and caprine cheeselike systems coagulated with enzymes from the plant Cynara cardunculus, were assessed. Prior to the assay, the 3,000-Da permeate from 45-d-old cheeselike systems was fractionated by tandem chromatographic techniques. Several peaks were obtained in each chromatogram, but only some were associated with ACE-inhibitory or antioxidant activity or both. Peptides Tyr-Gln-Glu-Pro, Val-Pro-Lys-Val-Lys, and Tyr-Gln-Glu-Pro-Val-Leu-Gly-Pro-* from beta-casein, as well as Arg-Pro-Lys and Arg-Pro-Lys-His-Pro-Ile-Lys-His-* from alpha(s1)-casein exhibited ACE-inhibitory activity. Peptides released upon cleavage of the peptide bond Leu190-Tyr191 (either in ovine or caprine beta-casein), and corresponding to the beta-casein sequence Tyr-Gln-Glu-Pro-*, possessed antioxidant activity.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9