Biological and physical-chemical characterization of phase pure HA and Si-Substituted hydrosyapatite by different microscopy techniques

• Type: Article in International Scientific Journal
• Date: 2004

Authors

Cláudia M. Botelho (Author) Other; R. A. Brooks (Author) Other; Maria Ascensão Lopes (Author) FEUP; Serena M. Best (Author) Other; José Domingos Santos (Author) FEUP; W. Bonfield (Author) Other

Journal

Title: Key Engineering Materials

Pages: 845-848

ISSN: 1013-9826

Publisher: Trans Tech Publications Ltd

Indexing

ISI Web of Science

Scientific classification

FOS: Engineering and technology > Medical engineering

Other information

DOI: 10.4028/www.scientific.net/KEM.254-256.845

Abstract (EN): Two different microscopy techniques were used to investigate the response of human osteoblasts to hydroxyapatite (HA) and silicon substituted hydroxyapatite (Si-HA), namely, fluorescence and confocal microscopy The changes in the surface of HA and Si-HA, after incubation for different periods of time in simulated body fluid, were assed using atomic force microscopy and environmental electron scanning microscopy. Cell proliferation was higher on Si-HA compared to HA. In addition more focal points of adhesion were seen in Si-HA than on HA. Using atomic force microscopy and environmental scanning electron microscopy it was possible to observe changes in the surface of both materials, namely dissolution features and the formation of an apatite layer. These findings support the results of a previous study, which showed that Si-HA had a higher dissolution with the preferential release of silicon into the medium and this fact may account for the changes observed in the cell behaviour.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 4