A Disposable Saliva Electrochemical MIP-Based Biosensor for Detection of the Stress Biomarker ¿-Amylase in Point-of-Care Applications

• Type: Article in International Scientific Journal
• Date: 2021

Authors

Rebelo T.S.C.R. (Author) Other; Miranda I.M. (Author) Other; Brandão A.T.S.C. (Author) FCUP; Sousa L.I.G. (Author) Other; José A. Ribeiro (Author) FCUP; Silva, F (Author) FCUP; Carlos M Pereira (Author) FCUP

Journal

The Journal is awaiting validation by the Administrative Services.

Title: Electrochem

Vol. 15

Pages: 427-438

Indexing

Scopus - 31 Citations

Other information

Authenticus ID: P-00V-S7N

DOI: 10.3390/electrochem2030028

Abstract (EN): The design and synthesis of artificial receptors based on molecular imprinting (MI) technology for the development of a new MIP-based biosensor for detection of the stress biomarker ¿-amylase in human saliva in point-of-care (PoC) applications is described in this work. The portable electrochemical devices for monitoring ¿-amylase consists of cost-effective and disposable gold screen-printed electrodes (AuSPEs). To build the electrochemical device, the template biomolecule was firstly immobilized directly over the working area of the gold chip previously activated with a self-assembled monolayer (SAM) of cysteamine (CA). Then, pyrrole (Py) monomer was selected as building block of a polymeric network prepared by CV electropolymerization. After the electropolymerization process, the enzyme was removed from the polymer film in order to build the specific recognition sites for the target enzyme. The MIP biosensor showed a very wide linear concentration range (between 3.0 × 10¿4 to 0.60 mg mL¿1 in buffer solution and between 3.0 × 10¿4 to 3.0 × 10¿2 mg mL¿1 in human saliva) and low detection levels were achieved (LOD < 3.0 × 10¿4 mg mL¿1) using square wave voltammetry (SWV) as the electroanalytical technique.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 11