beta-Carotene and its physiological metabolites: Effects on oxidative status regulation and genotoxicity in in vitro models

• Type: Article in International Scientific Journal
• Date: 2020

Authors

Ribeiro, D (Author) Other; Sousa, A (Author) Other; Nicola, P (Author) Other; de Oliveira, JMPF (Author) FCUP; Rufino, AT (Author) Other; Silva, M (Author) Other; Freitas, M (Author) Other; Felix Carvalho (Author) FFUP; Eduarda Fernandes (Author) FFUP

Journal

Title: Food and Chemical Toxicology

Vol. 141

ISSN: 0278-6915

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 6 Citations

Scopus - 6 Citations

Other information

Authenticus ID: P-00S-1J3

DOI: 10.1016/j.fct.2020.111392

Abstract (EN): Carotenoids are ubiquitously distributed in nature, beta-carotene being the most frequently found carotenoid in the human diet. In the human body, beta-carotene is absorbed, distributed and metabolized by enzymatic and/or nonenzymatic oxidant cleavage into several metabolites. Despite the broadly accepted biological value of beta-carotene, it has also been considered a double-edged sword, mainly due to its potential antioxidant versus prooxidant behaviour. In this sense, the aim of this work was to scrutinize the antioxidant or pro-oxidant potential of beta-carotene and its metabolites, namely trans-beta-apo-8'-carotenal and beta-ionone. Several parameters were evaluated in this study, viz. their effects on reactive species production, both in human whole blood and neutrophils; their effects on lipid peroxidation, in the absence and presence of peroxynitrite anion (ONOO-) or hydrogen peroxide (H2O2), using a synaptosomal model; and finally, their putative genotoxic effects in the human hepatic HepG2 cell line. In general, depending on the cellular model and conditions tested, beta-carotene and its metabolites revealed antioxidant effects to varying degrees without significant pro-oxidant or genotoxic effects.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9