Reproductive hormones affect follicular cells and ooplasm of Stage I and II oocytes in zebrafish

• Type: Article in International Scientific Journal
• Date: 2016

Authors

Sousa, ML (Author) Other; Silva, A (Author) Other; Malhao, F (Author) Other; rocha, mj (Author) ICBAS; rocha, e (Author) ICBAS; Urbatzka, R (Author) Other

Journal

Title: Reproduction, Fertility and Development

Vol. 28

Pages: 1945-1952

ISSN: 1031-3613

Publisher: CSIRO Publishing

Indexing

ISI Web of Knowledge - 0 Citations

Scopus - 1 Citation

Other information

Authenticus ID: P-00H-MJH

DOI: 10.1071/rd15100

Abstract (EN): The basic pathway of oocyte development and its regulation is evolutionarily conserved among vertebrates; however, little is known about the role of hormones at the first stages (Stages I and II) of follicle development in fish. In the present study, zebrafish follicles at Stages I and II were exposed in vitro to the reproductive hormones 17-oestradiol (E-2), 11-ketotestosterone (11KT), 17,20-dihydroxy-4-pregnen-3-one (DHP) and to the secondary messenger dibutyryl cyclic adenosine monophosphate (db-cAMP) at a concentration of 1 mu M for a 48-h period. Morphological alterations of the ooplasm were assessed by transmission electron microscopy and of the granulosa cell layer by quantitative stereology. Expression of mRNA was analysed for cell-cycle genes (cyclin B and E) and resident proteins of the endoplasmic reticulum (calnexin and 78-kDa glucose-regulated protein (grp78/bip)). E-2 and db-cAMP stimulated the presence of endoplasmic reticulum in the ooplasm and calnexin mRNA increased in the db-cAMP treatment, but also in response to 11KT and DHP. 11KT, DHP and db-cAMP inhibited the progression of the cell cycle in the granulosa-theca cell layer, indicated by a reduction of the nucleus volume-weighted size of granulosa cells and of increased cyclin E mRNA expression. Reproductive hormones had different effects on the ooplasm and the granulosa-theca cell layer of zebrafish follicles, predominantly at Stage II.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8