Immunomagnetic bead-based bioassay for the voltammetric analysis of the breast cancer biomarker HER2-ECD and tumour cells using quantum dots as detection labels

• Type: Article in International Scientific Journal
• Date: 2020

Authors

Freitas, M (Author) Other; Nouws, HPA (Author) Other; Elisa Keating (Author) FMUP; Fernandes, VC (Author) Other; Delerue Matos, C (Author) Other

Journal

Title: Mikrochimica Acta

Vol. 187

Final page: 184

ISSN: 0026-3672

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 39 Citations

Scopus - 39 Citations

Other information

Authenticus ID: P-00R-SSY

DOI: 10.1007/s00604-020-4156-4

Abstract (EN): An electrochemical magnetic immunosensing strategy was developed for the determination of HER2-ECD, a breast cancer biomarker, and breast cancer cells in human serum. A sandwich assay was performed on carboxylic acid-functionalized magnetic beads (MBs) using a screen-printed carbon electrode (SPCE) as transducer surface. The affinity process was detected using electroactive labels; core/shell streptavidin-modified CdSe@ZnS Quantum Dots (QDs). Cd2+ ions, released from the QDs, were determined by differential pulse anodic stripping voltammetry (DPASV). An assay time of 90 min, with an actual hands-on time of about 20 min, a linear range between 0.50-50 ng center dot mL(-1) of HER2-ECD and a limit of detection of 0.29 ng center dot mL(-1) were achieved. Analysis of live breast cancer cells was also performed using the optimized assay. Breast cancer cell lines SK-BR-3 (a HER2-positive cell line), MDA-MB-231 (a HER2-negative cell line) and MCF-7 (a cell line with low HER2 expression) were tested. The selectivity of the assay towards SK-BR-3 cells was confirmed. A concentration-dependent signal that was 12.5x higher than the signal obtained for the HER2-negative cells (MDA-MB-231) and a limit of detection of 2 cells center dot mL(-1) was obtained. Schematic representation of the electrochemical immunomagnetic assay for the determination of the breast cancer biomarker HER2-ECD and cancer cells using magnetic beads (MBs), a screen-printed carbon electrode (SPCE) as transducer surface and quantum dots (QD) as electroactive labels.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10