LC-MS and microscale NMR analysis of luciferin-related compounds from the bioluminescent earthworm Fridericia heliota

• Type: Article in International Scientific Journal
• Date: 2011

Authors

marques, sm (Author) Other; petushkov, vn (Author) Other; rodionova, ns (Author) Other; da silva, jcge (Author) FCUP

Journal

Title: Journal of Photochemistry and Photobiology B: Biology

Vol. 102 No. 3

Pages: 218-223

ISSN: 1011-1344

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 12 Citations

Scopus - 13 Citations

Scientific classification

FOS: Natural sciences > Biological sciences

Other information

Authenticus ID: P-002-TAJ

DOI: 10.1016/j.jphotobiol.2010.12.006

Abstract (EN): This paper presents the main results of RP-HPLC-MS and microscale NMR analysis performed on Accompanying similar to Luciferin (AsLn(x)), compounds present in extracts of the bioluminescent earthworm Fridericia heliota that display similarities with Fridericia's luciferin, the substrate of the bioluminescent reaction. Three isomers of AsLn were discovered, AsLn(1), AsLn(2) and AsLn(3), all of which present a molecular weight of 529 Da. Their UV-Vis absorption spectra show maxima at 235 nm for AsLn(1), 238 and 295 nm for AsLn(2) and 241 and 295 nm for AsLn(3). MSn fragmentation patterns suggest the existence of carboxylic acid and hydroxyl moieties, and possibly chemical groups found in other luciferins like pterin or benzothiazole. The major isomer, AsLn(2), presents an aromatic ring and alkene and alkyl moieties. These luciferin-like compounds can be used as models that could give further insights into the structure of this newly discovered luciferin.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6