Flavonoid metabolites transport across a human BBB model

• Type: Article in International Scientific Journal
• Date: 2014

Authors

Ana Faria (Author) FCUP; Manuela Meireles (Author) FMUP; Iva Fernandes (Author) FCUP; Celestino Santos Buelga (Author) Other; Susana Gonzalez Manzano (Author) Other; Montserrat Duenas (Author) Other; Victor de Freitas (Author) FCUP; Nuno Mateus (Author) FCUP; Conceicao Calhau (Author) FMUP

Journal

Title: Food Chemistry

Vol. 149

Pages: 190-196

ISSN: 0308-8146

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 104 Citations

Scopus - 118 Citations

Scientific classification

FOS: Agrarian Sciences > Other Agrarian Sciences

Other information

Authenticus ID: P-008-GSJ

DOI: 10.1016/j.foodchem.2013.10.095

Abstract (EN): This study aimed to evaluate the transmembrane transport of different flavonoids (flavan-3-ols, anthocyanins and flavonols) and some of their metabolites (methylated and conjugated with glucuronic acid) across hCMEC/D3 cells (a blood-brain barrier (BBB) model). Further metabolism of the tested compounds was assayed and their transport modulated in an attempt to elucidate the mechanisms behind this process. The transport across hCMEC/D3 cells was monitored in basolateral media at 1, 3 and 18 h by HPLC-DAD/MS. All the flavonoids and their metabolites were transported across hCMEC/D3 cells in a time-dependent manner. In general, the metabolites showed higher transport efficiency than the native flavonoid. No further biotransformation of the metabolites was found as consequence of cellular metabolism. Anthocyanins and their metabolites crossed this BBB cell model in a lipophilicity-dependent way. Quercetin transport was influenced by phosphatase modulators, suggesting a phosphorylation/dephosphorylation regulation mechanism. Overall, this work suggests that flavonoids are capable of crossing the BBB and reaching the central nervous system.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7