A microparticle blood analogue suspension matching blood rheology

• Type: Article in International Scientific Journal
• Date: 2021

Authors

J. Carneiro (Author) Other; R. Lima (Author) Other; João Bernardo L. M. de Campos (Author) FEUP; João Mário R. Miranda (Author) FEUP

Journal

Title: Soft Matter

Vol. 17

Pages: 3963-3974

ISSN: 1744-683X

Publisher: Royal Society of Chemistry

Indexing

ISI Web of Knowledge - 2 Citations

Scopus - 2 Citations

Other information

Authenticus ID: P-00T-THT

DOI: 10.1039/d1sm00106j

Abstract (EN): The handling of blood in vitro is demanding because of ethical, economical and safety issues. Although several Newtonian and non-Newtonian blood analogues are found in the literature, few studies have used particles to mimic red blood cells (RBCs) and built an analogue with similar rheological properties of blood. This work reports the development of a blood analogue suspension composed of polydimethylsiloxane (PDMS) microparticles with an average diameter of similar to 7 mu m. High throughput production of PDMS particles is possible using a multi-stage membrane emulsification process; up to similar to 6 mL of microparticles are manufactured in 3 hours. PDMS particles at a concentration of around 21% (w/w) at 20 degrees C present steady, oscillatory and extensional rheologies very similar to those of blood under physiological conditions (37 degrees C and similar to 41% hematocrit), making them a good candidate whole blood analogue. Also, flow studies were performed in microchannels with contraction to study the cell-free layer (CFL) formation and particle deformation, achieving good qualitative results. Using the procedure developed, it is possible to obtain blood analogue fluids with a shelf life of at least 6 months.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 12