Size exclusion and reversed-phase high-performance liquid chromatography/UV for routine control of thermal processing of cows' and donkey milk major proteins

• Type: Article in International Scientific Journal
• Date: 2012

Authors

Carina Pinho (Author) Other; Zita E Martins (Author) Other; Catarina Petisca (Author) Other; Agata M Figurska (Author) Other; Olivia Pinho (Author) FCNAUP; Isabel M P L V O Ferreira (Author) FFUP

Journal

Title: Journal of Dairy Research

Vol. 79 No. 2

Pages: 224-231

ISSN: 0022-0299

Publisher: Cambridge University Press

Indexing

ISI Web of Knowledge - 3 Citations

ISI Web of Science

Scopus - 3 Citations

Scientific classification

FOS: Agrarian Sciences > Animal and Dairy science

CORDIS: Health sciences

Other information

Authenticus ID: P-002-APS

DOI: 10.1017/s0022029912000064

Abstract (EN): Cows' and donkey milks (raw and thermally processed) and respective whey were analysed for quantification of major proteins. Two different chromatographic approaches, size exclusion (SE-HPLC) and reversed-phase high performance liquid chromatography (RP-HPLC) both coupled to UV detection were used. Usefulness of these methods for routine control of the effect of thermal processing was evaluated. The external standard method was used to calibrate the SE-HPLC and RP-HPLC systems. Concerning quantification of beta-lactoglobulin (beta-lg), alpha-lactalbumin (alpha-la), lysozyme (lys), and total casein (cn), no significant differences between results obtained by SE-HPLC and by RP-HPLC (t-test, P>0.05) were observed for raw milks and whey. Heating of cows' milk promoted aggregation of denatured proteins as observed by SE-HPLC, whereas alpha-la and beta-lg from donkey milk were stable to thermal processing at 100 degrees C (5 min). Lys was quantified in donkey raw milk and whey however, in thermally processed donkey milk lys was denatured and could not be quantified by HPLC.

Language: English

Type (Professor's evaluation): Scientific

Notes: <a href="http://gateway.isiknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=Alerting&SrcApp=Alerting&DestApp=WOS&DestLinkType=FullRecord&KeyUT=000303169200013">Indexado na ISI Web of Science</a>

No. of pages: 8