Modulation of insulin transport in rat brain microvessel endothelial cells by an ecto-phosphatase activity

• Type: Article in International Scientific Journal
• Date: 2002

Authors

Conceicao Calhau (Author) FCNAUP; Martel, F (Author) FMUP; Pinheiro Silva, S (Author) Other; Pinheiro, H (Author) Other; soares-da-silva, p (Author) FMUP; Hipolito Reis, C (Author) Other; Azevedo, I (Author) Other

Journal

Title: Journal of Cellular Biochemistry

Vol. 84

Pages: 389-400

ISSN: 0730-2312

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 31 Citations

Scopus - 33 Citations

Other information

Authenticus ID: P-000-REE

DOI: 10.1002/jcb.10027

Abstract (EN): The physiological function of alkaline phosphatase (ALP) remains controversial. It was recently suggested that this membrane-bound enzyme has a role in the modulation of transmembranar transport systems into hepatocytes and Caco-2 cells. ALP activity expressed on the apical surface of blood-brain barrier cells, and its relationship with I-125-insulin internalization were investigated under physiological conditions using p-nitrophenylphosphate (p-NPP) as substrate. For this, an immortalized cell line of rat capillary cerebral endothelial cells (RBE4 cells) was used. ALP activity and I-125-insulin internalization were evaluated in these cells. The results showed that RBE4 cells expressed ALP, characterized by an ecto-oriented active site which was functional at physiological pH. Orthovanaclate (100 muM), an inhibitor of phosphatase activities, decreased both RBE4-ALP activity and I-125-insulin internalization. in the presence Of L-arginine (1 mM) or adenosine (100 muM) RBE4-ALP activity and I-125-insulin, internalization were significantly reduced. However, D-arginine (1 mM) had no significant effect. Additionally, RBE4-ALP activity and I-125-insulin internalization significantly increased in the presence of the bioflavonoid kaempferol (100 muM), of the phorbol ester PMA (80 nM), IBMX (1 mM) progesterone (200 and 100 muM), beta -estradiol (100 muM), iron (100 muM), or in the presence of all-trans retinoic acid (RA) (10 muM). The ALP inhibitor levamisole (500 muM) was able to reduce I-125-insulin internalization to 69.1 +/- 7.1% of control. Our data showed a positive correlation between ecto-ALP activity and I-125-insulin incorporation (r=0.82; P <0.0001) in cultured rat brain endothelial cells, suggesting that insulin entry into the blood-brain barrier may be modulated through ALP. (C) 2001 Wiley-Liss, Inc.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 12