Evaluation of 3-hydroxy-benzo[a]pyrene levels in Nile tilapia (Oreochromis niloticus) after waterborne exposure to Benzo[a]pyrene

• Type: Article in International Scientific Journal
• Date: 2011

Authors

Rey Salgueiro, L (Author) Other; Ferreira, M (Author) Other; reis-henriques, ma (Author) FCUP

Journal

Title: Toxicological and Environmental Chemistry

Vol. 93

Pages: 2040-2054

ISSN: 0277-2248

Publisher: Taylor & Francis

Indexing

ISI Web of Knowledge - 15 Citations

Scopus - 10 Citations

Other information

Authenticus ID: P-002-ZMV

DOI: 10.1080/02772248.2011.606108

Abstract (EN): 3-Hydroxy-benzo[a]pyrene (3-OH-BaP), a toxic compound with the ability to covalently bind with the macromolecules (proteins and DNA), is one of the major phenolic metabolites of benzo[a] pyrene (BaP). The purpose of this study was to evaluate the presence of 3-OH-BaP in the bile and plasma of Nile tilapia by HPLC with fluorescence detection, after waterborne exposure to BaP (10 and 100 mu g L-1). Metabolites were detected in bile and plasma, and conjugates of 3-OH-BaP (glucuronide and/or sulphate conjugates) were the majority forms in both biological fluids, glucuronide 3-OH-BaP being the main metabolite in bile. Our data suggest that extrahepatic tissues as intestine or gill are important in BaP metabolism and need to be the considered sources of metabolites released into the blood. Although, low levels of 3-OH-BaP in toxic form (free form) were detected in plasma, one cannot exclude the possibility of circulating the levels leading to adverse effects.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 15