Regeneration of critical-sized defects, in a goat model, using a dextrin-based hydrogel associated with granular synthetic bone substitute

• Type: Article in International Scientific Journal
• Date: 2021

Authors

Pereira, I (Author) Other; Pereira, JE (Author) Other; Maltez, L (Author) Other; Rodrigues, A (Author) Other; Rodrigues, C (Author) Other; Oliveira, M (Author) Other; Silva, DM (Author) Other; Caseiro, AR (Author) Other; Prada, J (Author) Other; Mauricio, AC (Author) Other; Santos, JD (Author) FEUP; Gama, M (Author) Other

Journal

Title: Regenerative Biomaterials

Vol. 8

ISSN: 2056-3418

Publisher: Oxford University Press

Indexing

ISI Web of Knowledge - 0 Citations

Scopus - 1 Citation

Other information

Authenticus ID: P-00T-241

DOI: 10.1093/rb/rbaa036

Abstract (EN): The development of injectable bone substitutes (IBS) have obtained great importance in the bone regeneration field, as a strategy to reach hardly accessible defects using minimally invasive techniques and able to fit to irregular topographies. In this scenario, the association of injectable hydrogels and bone graft granules is emerging as a well-established trend. Particularly, in situ forming hydrogels have arisen as a new IBS generation. An in situ forming and injectable dextrin-based hydrogel (HG) was developed, aiming to act as a carrier of granular bone substitutes and bioactive agents. In this work, the HG was associated to a granular bone substitute (Bonelike (R)) and implanted in goat criticalsized calvarial defects (14mm) for 3, 6 and 12 weeks. The results showed that HG improved the handling properties of the Bonelike (R) granules and did not affect its osteoconductive features, neither impairing the bone regeneration process. Human multipotent mesenchymal stromal cells from the umbilical cord, extracellular matrix hydrolysates and the pro-angiogenic peptide LLKKK18 were also combined with the IBS. These bioactive agents did not enhance the new bone formation significantly under the conditions tested, according to micro-computed tomography and histological analysis.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10