Metabolic Biomarkers of Prenatal Disorders: An Exploratory NMR Metabonomics Study of Second Trimester Maternal Urine and Blood Plasma

• Type: Article in International Scientific Journal
• Date: 2011

Authors

Diaz, SO (Author) Other; Pinto, J (Author) Other; Graca, G (Author) Other; Duarte, IF (Author) Other; Barros, AS (Author) Other; Galhano, E (Author) Other; Pita, C (Author) Other; Almeida, MD (Author) Other; Goodfellow, BJ (Author) Other; Carreira, IM (Author) Other; Gil, AM (Author) Other

Journal

Title: Journal of Proteome Research

Vol. 10

Pages: 3732-3742

ISSN: 1535-3893

Publisher: American Chemical Society

Indexing

ISI Web of Knowledge - 147 Citations

Scopus - 173 Citations

Other information

Authenticus ID: P-002-P9F

DOI: 10.1021/pr200352m

Abstract (EN): This work describes an exploratory NMR metabonomic study of second trimester maternal urine and plasma, in an attempt to characterize the metabolic changes underlying prenatal disorders and identify possible early biomarkers. Fetal malformations have the strongest metabolic impact in both biofluids, suggesting effects due to hypoxia (leading to hypoxanthine increased excretion) and a need for enhanced gluconeogenesis, with higher ketone bodies (acetone and 3-hydroxy-butyric acid) production and TCA cycle demand (suggested by glucogenic amino acids and cis-aconitate overproduction). Choline and nucleotide metabolisms also seem affected and a distinct plasma lipids profile is observed for mothers with fetuses affected by central nervous system malformations. Urine from women who subsequently develop gestational diabetes mellitus exhibits higher 3-hydroxyisovalerate and 2-hydroxyisobutyrate levels, probably due to altered biotin status and amino acid and/or gut metabolisms (the latter possibly related to higher BMI values). Other urinary changes suggest choline and nucleotide metabolic alterations, whereas lower plasma betaine and TMAO levels are found. Chromosomal disorders and pre-preterm delivery groups show urinary changes in choline and, in the latter case, in 2-hydroxyisobutyrate. These results show that NMR metabonomics of maternal biofluids enables the noninvasive detection of metabolic changes associated to prenatal disorders, thus unveiling potential disorder biomarkers.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 11