Effect of process parameters on production of a biopolymer by Rhizobium sp.

• Type: Article in International Conference Proceedings Book
• Date: 2004

Authors

Duta, FP (Author) Other; De Franca, FP (Author) Other; Servulo, EFC (Author) Other; Lopes, LMD (Author) Other; Da Costa, ACA (Author) Other; Barros, A (Author) Other

Conference proceedings International

Title: APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

Pages: 639-652

25th Symposium on Biotechnology for Fuels and Chemicals

Breckenridge, CO, MAY 04-07, 2003

Indexing

ISI Web of Knowledge - 14 Citations

Scopus - 17 Citations

Other information

Authenticus ID: P-000-B9N

DOI: 10.1385/abab:114:1-3:639

Abstract (EN): The production of biopolymers by a Rhizobium strain was studied under batch and bioreactor conditions. The best viscosity levels were obtained under low mannitol concentrations as well as low agitation and aeration conditions. Infrared spectra indicated the presence of chemical groups characteristic of microbially produced biopolymers, including C = O and O-acetyl groups. Thermogravimetric analysis showed the characteristic degradation profiles of the exopolysaccharide produced (T-onset = 290degreesC). The experimental design showed that a low substrate concentration (10.0 g/L), and low aeration (0.2 vvm) and agitation (200 rpm) levels should be used. The maximum yield of the process was a Yp/s (g/g) of 0.19 +/- 0.1, obtained under optimized conditions.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 14