Transient HES5 Activity Instructs Mesodermal Cells toward a Cardiac Fate

• Type: Article in International Scientific Journal
• Date: 2017

Authors

Freire, AG (Author) Other; Waghray, A (Author) Other; Soares da Silva, F (Author) Other; Resende, TP (Author) Other; Lee, DF (Author) Other; Pereira, CF (Author) Other; Nascimento, DS (Author) ICBAS; Lemischka, IR (Author) Other; Perpetua Pinto do O (Author) ICBAS

Journal

Title: Stem Cell Reports

Vol. 9

Pages: 136-148

ISSN: 2213-6711

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 3 Citations

Scopus - 3 Citations

Other information

Authenticus ID: P-00R-AQ2

DOI: 10.1016/j.stemcr.2017.05.025

Abstract (EN): Notch signaling plays a role in specifying a cardiac fate but the downstream effectors remain unknown. In this study we implicate the Notch downstream effector HES5 in cardiogenesis. We show transient Hes5 expression in early mesoderm of gastrulating embryos and demonstrate, by loss and gain-of-function experiments in mouse embryonic stem cells, that HES5 favors cardiac over primitive erythroid fate. Hes5 overexpression promotes upregulation of the cardiac gene Isl1, while the hematopoietic regulator Scl is downregulated. Moreover, whereas a pulse of Hes5 instructs cardiac commitment, sustained expression after lineage specification impairs progression of differentiation to contracting cardiomyocytes. These findings establish a role for HES5 in cardiogenesis and provide insights into the early cardiac molecular network.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 13