Substrate effect on bacterial communities from constructed wetlands planted with Typha latifolia treating industrial wastewater

• Type: Article in International Scientific Journal
• Date: 2009

Authors

Calheiros, CSC (Author) Other; Duque, AF (Author) Other; Moura, A (Author) Other; Henriques, IS (Author) Other; Correia, A (Author) Other; Rangel, AOSS (Author) Other; Castro, PML (Author) Other

Journal

Title: Ecological Engineering

Vol. 35

Pages: 744-753

ISSN: 0925-8574

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 47 Citations

Scopus - 48 Citations

Other information

Authenticus ID: P-003-KA3

DOI: 10.1016/j.ecoleng.2008.11.010

Abstract (EN): Constructed wetlands (CWs) have been recognized as being able to effectively treat wastewater from municipal and industrial sources. This study focused on the effect of different substrates and long-term operation of horizontal subsurface flow CWs treating tannery wastewater on the bacterial communities. The CWs were planted with Typha latifolia in three types of substrate: two units with different types of expanded clay aggregates and one unit with fine gravel. Another unit with expanded clay was left unvegetated. Changes in the bacterial community related to the type of substrate, different hydraulic loading rates and along CW operation were examined using denaturating gradient gel electrophoresis (DGGE). Bacterial enumeration was also per-formed and several bacterial isolates were retrieved from the CWs. Phylogenetic affiliations of those isolates were obtained on the basis of 16S rRNA gene sequences and revealed that they were closely related to the genera Bacillus (TM1S1, TM1R3, TNR1 and TAR1), Paracoccus (TM1R2), Pseudomonas (TM1R1) and Halomonas (TM1S2). The type of substrate and the presence of T latifolia had a major effect on the species richness and the structure of bacterial communities as inferred by numerical analysis of DGGE profiles.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10