L-proline supplementation improves nitric oxide bioavailability and counteracts the blood pressure rise induced by angiotensin II in rats

• Type: Article in International Scientific Journal
• Date: 2019

Authors

Leal, J (Author) Other; Teixeira Santos, L (Author) Other; Pinho, Dora (Author) FMUP; Afonso, J (Author) FMUP; Carvalho, J (Author) Other; Maria de Lourdes Bastos (Author) FFUP; Albino Teixeira, A (Author) Other; Fraga, S (Author) Other; Sousa, T (Author) FMUP

Journal

Title: Nitric Oxide - Biology and Chemistry

Vol. 82

Pages: 1-11

ISSN: 1089-8603

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 20 Citations

Scopus - 24 Citations

Other information

Authenticus ID: P-00P-X2E

DOI: 10.1016/j.niox.2018.10.007

Abstract (EN): We evaluated whether L-proline (Pro) supplementation improves redox status and nitric oxide (NO) bioavailability and prevents or delays angiotensin II (AngII)-induced hypertension. Male Sprague-Dawley rats were distributed to four experimental groups: Pro + AngII (Pro-Ang), Pro + Saline (Pro-Sal), Vehicle + AngII (Veh-Ang) and Veh + Saline (Veh-Sal). Pro solution (2 g.kg(-1).day(-1)) or water (vehicle) were orally administered, from day 0 to day 21. AngII (200 ng.kg(-1).min(-1)) or saline were infused (s.c.) from day 7 to day 21. Systolic blood pressure (SBP) was measured by the tail-cuff method. From day 20-21, animals were kept on metabolic cages for 24h-urine collection. On day 21, urine and blood were collected for further quantification of redox status biomarkers, NO-related markers (urinary nitrates and nitrites, U-NOx; plasma asymmetric dimethylarginine, P-ADMA), metabolic and renal parameters. Pro prevented the AngII-induced SBP rise [mean (95% CI), Day 19: Pro-AngII, 137 (131; 143) vs. Veh-AngII, 157 (151; 163) mm Hg, P < 0.001]. Pro-AngII rats also had increased values of U-NOx, systemic and urinary total antioxidant status (TAS), urinary H2O2 and plasma urea, as well as reduced P-ADMA and unaltered urinary isoprostanes. Plasma Pro was inversely correlated with P-ADMA (r = -0.52, p = 0.0009) and positively correlated with urinary TAS (r = 0.55, p = 0.0005) which, in turn, was inversely correlated with P-ADMA (r = -0.56, p = 0.0004). Furthermore, urinary H2O2 values decreased across P-ADMA tertiles (p for linear trend = 0.023). These results suggest that Pro reduces P-ADMA levels and improves redox status, thereby increasing NO bioavailability and counteracting the AngII-induced SBP rise. H2O2 and TAS modulation by Pro may contribute to the reduced P-ADMA concentration.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 11