Dynamics of clonal and plasmid backgrounds of Enterobacteriaceae producing acquired AmpC in Portuguese clinical settings over time

• Type: Article in International Scientific Journal
• Date: 2019

Authors

Ribeiro, TG (Author) Other; Novais, A (Author) Other; Rodrigues, C (Author) Other; Nascimento, R (Author) Other; Freitas, F (Author) Other; Machado, E (Author) Other; Luisa Peixe (Author) FFUP

Journal

Title: International Journal of Antimicrobial Agents

Vol. 53

Pages: 650-656

ISSN: 0924-8579

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 0 Citations

Scopus - 8 Citations

Other information

Authenticus ID: P-00Q-FE7

DOI: 10.1016/j.ijantimicag.2019.03.013

Abstract (EN): Objectives: The objective of this work was to provide detailed molecular data on clinically acquired AmpC (qAmpC)-producing Enterobacteriaceae from two different periods (2002-2008 and 2010-2013) in order to clarify the contribution of clonal and plasmid genetic platforms for the current epidemiological scenario concerning extended-spectrum beta-lactams resistance. Methods: We analysed 1246 Enterobacteriaceae non-susceptible to third-generation cephalosporins from two hospitals and one community laboratory between 2010 and 2013. Bacterial identification, antibiotic susceptibility, identification of qAmpC and plasmid-mediated quinolone resistance genes, clonal (pulsed-field gel electrophoresis (PFGE), Multilocus sequence typing (MLST)) and plasmid (S1-/I-CeuI-PFGE, replicon typing, hybridization) analysis were performed by standard methods. Whole-genome sequencing (WGS) was performed in two ST11-Klebsiella pneumoniae isolates harbouring DHA-1. Results: The occurrence of qAmpC was lower (2.6%) than that observed in a previous survey (7.4%), and varied slightly over time. Isolates produced DHA-1 (53%), CMY-2 (44%) or DHA-6 (3%), but significant epidemiological changes were observed in the two surveys. While DHA-1 persisted in different institutions by selection of a worldwide epidemic IncR plasmid in an ST11 harbouring KL105, CMY-2 rates increased over time linked to IncI1 plasmids (instead of IncK or IncA/C-2) in multiple Escherichia coli clones. Conclusions: The higher frequency of DHA-1 qAmpC in these species contrasts with the scenario in most European countries. Furthermore, the different genetic backgrounds associated with either extended-spectrum beta-lactamases (ESBLs) or acquired AmpC beta-lactamases (qAmpC) in our country might have contributed to their differential expansion.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7