ABC transporters, CYP1A and GST alpha gene transcription patterns in developing stages of the Nile tilapia (Oreochromis niloticus)

• Type: Article in International Scientific Journal
• Date: 2012

Authors

reis-henriques, ma (Author) Other; Filipe F C Castro (Author) ICBAS; Ferreira, M (Author) Other

Journal

Title: Gene

Vol. 506

Pages: 317-324

ISSN: 0378-1119

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 9 Citations

Scopus - 11 Citations

Other information

Authenticus ID: P-002-5FQ

DOI: 10.1016/j.gene.2012.06.092

Abstract (EN): In fish, some ABC transporters are implicated in a multixenobiotic resistance (MXR) mechanism to deal with the presence of xenobiotics, by effluxing them, or their metabolites, from inside the cells. These efflux transporters have been considered an integral part of cellular detoxification pathways, acting in coordination with phase I and II detoxification enzymes. However, the full characterization of this detoxification system is still incomplete, especially during the developmental stages of aquatic organisms, which are particularly sensitive periods to the presence of anthropogenic contamination. The goal of this study was to evaluate the mRNA expression dynamics of putatively important MXR proteins (ABCB1b, ABCB11, ABCC1, ABCC2 and ABCG2a) and phase I (CYP1A) and II (GST alpha) biotransformation enzymes, during the embryonic and larval developments of the specie Oreochromis niloticus (Nile tilapia). Our results showed that ABCB1b, ABCC1, CYP1A and GST alpha transcripts are maternally transmitted. Transcripts for ABCB11, ABCC2 and ABCG2a were only detected after the pharyngula period, which precedes a highly sensitive stage in the embryonic development, the hatching. This study has shown, for the first time, very distinct expression patterns of genes encoding for proteins involved in protection mechanisms against pollutants during the development of Nile tilapia. Moreover, the temporal pattern of gene expression suggests that increased intrinsic protection levels are required at specific developmental stages.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8