Extraction of phospholipid-rich fractions from egg yolk and development of liposomes entrapping a dietary polyphenol with neuroactive potential

• Type: Article in International Scientific Journal
• Date: 2019

Authors

Bernardo, J (Author) Other; Videira, RA (Author) Other; Patricia Valentao (Author) FFUP; Veiga, F (Author) Other; Paula B Andrade (Author) FFUP

Journal

Title: Food and Chemical Toxicology

Vol. 133

ISSN: 0278-6915

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 0 Citations

Scopus - 5 Citations

Other information

Authenticus ID: P-00Q-XHD

DOI: 10.1016/j.fct.2019.110749

Abstract (EN): A new protocol to obtain egg yolk phospholipids in ethanol is presented. Rutin-phospholipids nanoliposomes were prepared and characterized. The procedure takes advantage of the different solubility of egg yolk lipids in ethanol and acetone at low temperature, to efficiently obtain a phospholipid-rich fraction of high purity degree. The phospholipid content in the final fraction is 208.65 +/- 26.46 mu mol/g fresh egg yolk (16%), accounting for ca. 96% of the extract's dry weight. The phospholipid-rich fraction contains cholesterol (0.069-0.082 cholesterol/phospholipid molar ratio), and vestigial amounts of lutein and zeaxanthin (89.24 +/- 9.76 and 14.9 +/- 2.16 ng/g of fresh egg yolk, respectively). Saturated fatty acids dominate the extracted phospholipids (50% of eggs total yolk phospholipids), the levels of monounsaturated ranging from 20 to 25%, and polyunsaturated up to 35%. Rutin-liposomes, prepared with phospholipid-rich fraction, presented mean diameter < 140 nm, negative surface charge (Zeta potential similar to - 13 mV), and entrapment efficiency of rutin up to 87%. In human neuroblastoma cell line SH-SY5Y, rutin-liposomes (lipid 25 mu M + rutin 16.7 mu M) attenuated glutamate-induced cytotoxicity, in part by reducing the formation of intracellular reactive species, pointing to their potential application as new functional neuroprotective agents.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 11