Biodistribution and metabolic profile of 3,4-dimethylmethcathinone (3,4-DMMC) in Wistar rats through gas chromatography-mass spectrometry (GC-MS) analysis

• Type: Article in International Scientific Journal
• Date: 2020

Authors

Rouxinol, D (Author) Other; Dias da Silva, DCD (Author) Other; Teixeira, C (Author) Other; Felix Carvalho (Author) FFUP; Silva, JP (Author) Other; Carvalho, FD (Author) Other; Bastos, MDL (Author) Other; Helena Carmo (Author) FFUP

Journal

Title: Toxicology Letters

Vol. 320

Pages: 113-123

ISSN: 0378-4274

Publisher: Elsevier

Indexing

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Scopus - 5 Citations

Other information

Authenticus ID: P-00R-2K2

DOI: 10.1016/j.toxlet.2019.10.003

Abstract (EN): 3,4-Dimethylmethcathinone (3,4-DMMC) is a new psychoactive substance whose recreational use and trade have recently increased. Given the absence of information on the toxicokinetics of 3,4-DMMC, the present work aimed at validating a GC-MS methodology for the drug quantification in biological matrices, and further characterizing its biodistribution in Wistar rats. The method was validated based on the evaluation of the drug stability, limit of detection and quantification, linearity, selectivity, precision, accuracy and recovery. To characterize biodistribution, Wistar rats were administered with 20 or 40 mg/Kg of 3,4-DMMC i.p.. After 1 h or 24 h, rats were anaesthetized, euthanized and blood, brain, liver, heart, kidneys, lungs, spleen, urine (only at 24 h), and a portion of gut, muscle and adipose tissue were collected for analysis. After 1 h, 3,4-DMMC was present in all analysed matrices, and the presence of two metabolites was further detected in all of them. The drug accumulation was higher in kidneys, lungs, spleen and brain. After 24 h, 3,4-DMMC was only present in urine, along with five metabolites. All metabolites were tentatively identified. Through elucidation of the most appropriate analytical matrices and the metabolites that may have the largest detection windows, these data are expected to assist in future clinical and forensic investigations.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 11