L-Theanine promotes cultured human Sertoli cells proliferation and modulates glucose metabolism

• Type: Article in International Scientific Journal
• Date: 2019

Authors

Dias, TR (Author) Other; Bernardino, RL (Author) Other; Alves, MG (Author) Other; Silva, J (Author) Other; barros, a (Author) FMUP; sousa, m (Author) ICBAS; Susana Casal (Author) FFUP; Silva, BM (Author) Other; Oliveira, PF (Author) FMUP

Journal

Title: European Journal of Nutrition

Vol. 58

Pages: 2961-2970

ISSN: 1436-6207

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 23 Citations

Scopus - 24 Citations

Other information

Authenticus ID: P-00Q-PQ1

DOI: 10.1007/s00394-019-01999-2

Abstract (EN): Purpose L-Theanine is the major free amino acid present in tea (Camellia sinensis L.). The effects of several tea constituents on male reproduction have been investigated, but l-theanine has been overlooked. Sertoli cells (SCs) are essential for the physical and nutritional support of germ cells. In this study, we aimed to investigate the ability of l-theanine to modulate important mechanisms of human SCs (hSCs) metabolism, mitochondrial function and oxidative profile, which are essential to prevent or counteract spermatogenesis disruption in several health conditions. Methods We evaluated the effect of a dose of l-theanine attained by tea intake (5 mu M) or a pharmacological dose (50 mu M) on the metabolism (proton nuclear magnetic resonance and Western blot), mitochondrial functionality (protein expression of mitochondrial complexes and JC1 ratio) and oxidative profile (carbonyl levels, nitration and lipid peroxidation) of cultured hSCs. Results Exposure of hSCs to 50 mu M of l-theanine increased cell proliferation and glucose consumption. In response to this metabolic adaptation, there was an increase in mitochondrial membrane potential, which may compromise the prooxidant-antioxidant balance. Still, no alterations were observed regarding the oxidative damages. Conclusions A pharmacological dose of l-theanine (50 mu M) prompts an increase in hSCs proliferation and a higher glucose metabolization to sustain the pool of Krebs cycle intermediates, which are crucial for cellular bioenergetics and biosynthesis. This study suggests an interplay between glycolysis and glutaminolysis in the regulation of hSCs metabolism.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10