Pandemic Escherichia coli ST648 isolate harbouring fosA3 and bla(CTX-M-8) on an IncI1/ ST113 plasmid: A new successful combination for the spread of fosfomycin resistance?

• Type: Another Publication in an International Scientific Journal
• Date: 2018

Authors

Palmeira, JD (Author) Other; Helena Sousa (Author) FFUP; Madec, JY (Author) Other; Haenni, M (Author) Other

Journal

Title: Journal of Global Antimicrobial Resistance

Vol. 15

Pages: 254-255

ISSN: 2213-7165

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 3 Citations

Scopus - 7 Citations

Other information

Authenticus ID: P-00P-X46

DOI: 10.1016/j.jgar.2018.10.025

Abstract (EN): Objectives: The emergence of Enterobacteriaceae isolates resistant to the last-resort antibiotic fosfomycin outside of Asia is a public-health issue. Here we report the draft genome of an Escherichia coli isolate presenting both an extended-spectrum beta-lactamase (ESBL) and the fosA3 gene in a healthy cow in Brazil. Methods: Whole genomic DNA from E. coli E12 was extracted and 2 x 150-bp paired-end reads were generated using Illumina sequencing technology. De novo genome assembly was performed using SPAdes v.3.11 and the draft genome was annotated by the NCBI Prokaryotic Genome Annotation Pipeline. Further analyses were performed using the Center for Genomic Epidemiology databases. Results: The 5 045 934-bp genome displayed several resistance genes, including the fosA3 and bla(CTX-M-8) genes. Southern blot experiments showed that they were co-located on an IncI1/ST113 plasmid. Conclusion: Presence of the fosA3 gene on the same common plasmid as bla(CTX-M-8) will have to be monitored. This draft genome provides data that will help in tracing the dissemination of this gene and the evolution of its plasmidic support.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 2