Clarifying the Catalytic Mechanism of Human Glutamine Synthetase: A QM/MM Study

• Type: Article in International Scientific Journal
• Date: 2017

Authors

Moreira, C (Author) Other; Ramos, MJ (Author) FCUP; Pedro A Fernandes (Author) FCUP

Journal

Title: Journal of Physical Chemistry B

Vol. 121

Pages: 6313-6320

ISSN: 1520-6106

Publisher: American Chemical Society

Indexing

ISI Web of Knowledge - 7 Citations

Scopus - 9 Citations

Other information

Authenticus ID: P-00N-G9F

DOI: 10.1021/acs.jpcb.7b02543

Abstract (EN): Glutamine synthetase (GS) is a crucial enzyme responsible for the elimination of both neurotoxic glutamate and toxic ammonium, by combining them into glutamine. Alterations on the GS activity are associated with severe liver and neurodegenerative diseases and its absence or malformation results in death. In this work, the catalytic mechanism of human GS has been investigated with high-level QM/MM calculations, showing a two-phase reaction cycle. During phase 1, GS activates the reactants (NH4+ and glutamate) with extreme efficiency, through NH4+ deprotonation by E305 and glutamate phosphorylation by ATP, in two spontaneous and barrierless reactions. At phase 2, NH3 attacks the gamma-glutamyl phosphate being concomitantly deprotonated by the leaving PO43-, forming the glutamine and HPO42- products. The second phase contains the rate limiting step, with a Delta G* of 19.2 kcal.mol(-1) associated with the nucleophilic substitution of the phosphate by NH3. The final reaction free energy is -34.5 kcal.mol(-1). Both phases are exergonic, the first by -22.9 kcal.mol(-1) and the second by -11.6 kcal.mol(-1). Direct NH4+ attack is shown to be inefficient; the possible bases that perform the NH4+ deprotonation were systematically investigated. Negative E305 was shown to be the only one possibly responsible for NH4+ deprotonation. Altogether, these results provide a clear atomic level picture of the reaction cycle of GS, consistent with experimental and theoretical studies on GS of this and other organisms, and provide the necessary insights for the development of more specific therapeutic GS inhibitors.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8