T cells in mesenteric and subcutaneous adipose tissue of Holstein-Friesian cows

• Type: Article in International Scientific Journal
• Date: 2019-03-04

Authors

Bárbara M. Oliveira (Author) ICBAS; Alexandra Correia (Author) ICBAS; Ana Pinto (Author) ICBAS; Pedro Meireles (Author) ICBAS; Paula G Ferreira (Author) ICBAS; Manuel Vilanova (Author) ICBAS; Luzia Teixeira (Author) ICBAS

Journal

Title: Scientific Reports

Vol. 9 No. 3413

ISSN: 2045-2322

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 4 Citations

Scopus - 6 Citations

Associated Projects

ADIPONEO - Estudo da resposta imunológica no tecido adiposo de hospedeiros infectados com Neospora Caninum

Other information

Authenticus ID: P-00Q-8PS

DOI: 10.1038/s41598-019-39938-0

Abstract (EN): The importance of immune cells present in the adipose tissue to metabolic homeostasis has been increasingly recognized. Nevertheless, in bovines few studies have so far addressed the immune cell populations resident in this tissue. Here we developed an eight-colour flow cytometry panel to address T cell populations present in bovine adipose tissue. Our results showed that gamma dT cells, CD4(+) and CD8(+) CD3(+) non-gamma dT cells, as well as NK cells, are present in the mesenteric and subcutaneous adipose tissue of Holstein-Friesian cows. The frequency of both gamma dT cells and CD8(+) non-gamma dT cells was found higher in mesenteric than in subcutaneous adipose tissue. The majority of T cells in adipose tissue presented a CD45RO(+)CD62L(-) phenotype, characteristic of effector memory cells, and the frequency of these cellular populations was higher than in the blood. The ratio of CD4(+) T cells over CD8(+) T cells was similar between subcutaneous and mesenteric adipose tissue but different from the one found in blood. Overall, our results highlight particular phenotypic characteristics of bovine adipose tissue T cell populations.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10