Structural polymeric features that contribute to in vitro immunostimulatory activity of instant coffee

• Type: Article in International Scientific Journal
• Date: 2018

Authors

Ferreira, SS (Author) Other; Passos, CP (Author) Other; Cepeda, MR (Author) Other; Lopes, GR (Author) Other; Teixeira Coelho, M (Author) Other; Madureira, P (Author) Other; Nunes, FM (Author) Other; Manuel Vilanova (Author) ICBAS; Coimbra, MA (Author) Other

Journal

Title: Food Chemistry

Vol. 242

Pages: 548-554

ISSN: 0308-8146

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 11 Citations

Scopus - 11 Citations

Other information

Authenticus ID: P-00N-1MS

DOI: 10.1016/j.foodchem.2017.09.059

Abstract (EN): An instant coffee fraction, rich in arabinogalactans, obtained by ultrafiltration, using 1 and 5 kDa membranes, has previously shown in vitro stimulatory activity on BALB/c mice spleen B lymphocytes. The compounds inducing lymphocytic activation were shown to elute, mainly near the void volume by size-exclusion chromatography, using Bio-Gel P6 (1-6 kDa). Treatment of the compounds with chymotrypsin, a digestive protease, did not affect the induced B lymphocyte activation. On the contrary, hydrolysis with an alpha-L-arabinofuranosidase, removing single terminally-linked arabinose residues, decreased the extent of B cell activation. The immunostimulatory activity of these compounds was also observed by in vitro experiments, using bone marrowderived macrophages and dendritic cells as responders. Altogether, these results show the relevance of single arabinose residues, present at the non-reducing end of polymeric compounds, to the coffee stimulatory activity in cells mediating innate and acquired immunity.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7