Aptamer-based fiber sensor for thrombin detection

• Type: Article in International Scientific Journal
• Date: 2016

Authors

Coelho, L (Author) FCUP; de Almeida, JMMM (Author) FCUP; Santos, JL (Author) FCUP; Jorge, PAS (Author) FCUP; Martins, MCL (Author) Other; Viegas, D (Author) FCUP; Queiros, RB (Author) Other

Journal

Title: Journal of Biomedical Optics

Vol. 21

Final page: 087005

ISSN: 1083-3668

Publisher: SPIE

Indexing

ISI Web of Knowledge - 38 Citations

Scopus - 41 Citations

Other information

Authenticus ID: P-00K-T77

DOI: 10.1117/1.jbo.21.8.087005

Abstract (EN): The detection of thrombin based on aptamer binding is studied using two different optical fiber-based configurations: long period gratings coated with a thin layer of titanium dioxide and surface plasmon resonance devices in optical fibers coated with a multilayer of gold and titanium dioxide. These structures are functionalized and the performance to detect thrombin in the range 10 to 100 nM is compared in transmission mode. The sensitivity to the surrounding refractive index (RI) of the plasmonic device is higher than 3100 nmRIU(-1) in the RI range 1.335 to 1.355, a factor of 20 greater than the sensitivity of the coated grating. The detection of 10 nM of thrombin was accomplished with a wavelength shift of 3.5 nm and a resolution of 0.54 nM. (C) 2016 Society of Photo-Optical Instrumentation Engineers (SPIE)

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9