Hybrid Microfluidic Platform for Multifactorial Analysis Based on Electrical Impedance, Refractometry, Optical Absorption and Fluorescence

• Type: Article in International Scientific Journal
• Date: 2016

Authors

Pereira, FM (Author) Other; Bernacka Wojcik, I (Author) Other; Ribeiro, RSR (Author) Other; Lobato, MT (Author) Other; Fortunato, E (Author) Other; Martins, R (Author) Other; Igreja, R (Author) Other; Jorge, PAS (Author) FCUP; Aguas, H (Author) Other; Gonzalez Oliva, AMG (Author) Other

Journal

Title: Micromachines

Vol. 7

Final page: 181

ISSN: 2072-666X

Publisher: MDPI

Indexing

ISI Web of Knowledge - 1 Citation

Scopus - 1 Citation

Other information

Authenticus ID: P-00M-6PF

DOI: 10.3390/mi7100181

Abstract (EN): This paper describes the development of a novel microfluidic platform for multifactorial analysis integrating four label-free detection methods: electrical impedance, refractometry, optical absorption and fluorescence. We present the rationale for the design and the details of the microfabrication of this multifactorial hybrid microfluidic chip. The structure of the platform consists of a three-dimensionally patterned polydimethylsiloxane top part attached to a bottom SU-8 epoxy-based negative photoresist part, where microelectrodes and optical fibers are incorporated to enable impedance and optical analysis. As a proof of concept, the chip functions have been tested and explored, enabling a diversity of applications: (i) impedance-based identification of the size of micro beads, as well as counting and distinguishing of erythrocytes by their volume or membrane properties; (ii) simultaneous determination of the refractive index and optical absorption properties of solutions; and (iii) fluorescence-based bead counting.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 15