Assessment of ionic liquids' toxicity through the inhibition of acylase I activity on a microflow system

• Type: Article in International Scientific Journal
• Date: 2017

Authors

Azevedo, AMO (Author) Other; Pereira, SAP (Author) Other; Passos, MLC (Author) Other; Costa, SPF (Author) Other; Pinto, PCAG (Author) Other; Araujo, ARTS (Author) Other; Lucia L M F S Saraiva (Author) FFUP

Journal

Title: Chemosphere

Vol. 173

Pages: 351-358

ISSN: 0045-6535

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 5 Citations

Scopus - 9 Citations

Other information

Authenticus ID: P-00M-D9R

DOI: 10.1016/j.chemosphere.2016.12.138

Abstract (EN): Acylase I (ACY I) plays a role in the detoxication and bioactivation of xenobiotics as well in other physiological functions. In this context, an automated ACY I assay for the evaluation of ionic liquids' (Ms) toxicity was developed. The assay was implemented in a sequential injection analysis (SIA) system and was applied to eight commercially available ILs. The SIA methodology was based on the deacetylation of N-acetyl-L-methionine with production of L-methionine, which was determined using fluorescamine. ACY I inhibition in the presence of ILs was monitored by the decrease of fluorescence intensity. The obtained results confirmed the influence of ILs' structural elements on its toxicity and revealed that pyridinium and phosphonium cations, longer alkyl side chains and tetrafluoroborate anion displayed higher toxic effect on enzyme activity. The developed methodology proved to be-robust and exhibited good repeatability (RSD < 1.3%, n = 10), leading also to a reduction of reagents consumption and effluents production. Thus, it is expected that the proposed assay can be used as a novel tool for ILs' toxicity screening.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8