Citrobacter portucalensis sp nov., isolated from an aquatic sample

• Type: Article in International Scientific Journal
• Date: 2017

Authors

Ribeiro, TG (Author) Other; Goncalves, BR (Author) Other; da Silva, MS (Author) Other; Novais, A (Author) Other; Machado, E (Author) Other; Carrico, JA (Author) Other; Luisa Peixe (Author) FFUP

Journal

Title: International Journal of Systematic and Evolutionary Microbiology

Vol. 67

Pages: 3513-3517

ISSN: 1466-5026

Publisher: Microbiology Society

Indexing

ISI Web of Knowledge - 6 Citations

Scopus - 16 Citations

Other information

Authenticus ID: P-00N-1SV

DOI: 10.1099/ijsem.0.002154

Abstract (EN): A Gram-stain-negative strain, A60(T), isolated from a water well sample in Portugal, was characterized phenotypically, genotypically and phylogenetically. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain A60(T) belonged to the genus Citrobacter, and recN gene phylogeny revealed one strongly supported clade encompassing strain A60(T) and 13 other strains from public databases, distinct from currently recognized species of the genus Citrobacter. Furthermore, multilocus sequence analysis (MLSA) based on concatenated partial fusA, leuS, pyrG and rpoB sequences confirmed the classification obtained with the recN sequence. In silico genomic comparisons, including average nucleotide identity (ANI) and the genome-to-genome distance calculator (GGDC), showed 94.6% and 58.4% identity to the closest relative Citrobacter freundii ATCC 8090(T), respectively. The ability to metabolize different compounds further discriminated strain A60(T) from other species of the genus Citrobacter. The G+C content of strain A60(T) is 52.0 %. The results obtained support the description of a novel species within the genus Citrobacter, for which the name Citrobacter portucalensis sp. nov. is proposed, with the type strain A60(T) (= DSM 104542(T)=CECT 9236(T)).

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 5