Factors influencing somatic embryogenesis induction in Eucalyptus globulus Labill.: basal medium and anti-browning agents

• Type: Article in International Scientific Journal
• Date: 2008

Authors

Pinto, G (Author) Other; Silva, S (Author) Other; Park, YS (Author) Other; Neves, L (Author) Other; Araujo, C (Author) Other; Santos, C (Author) Other

Journal

Title: Plant Cell, Tissue and Organ Culture

Vol. 95

Pages: 79-88

ISSN: 0167-6857

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 40 Citations

Scopus - 53 Citations

Other information

Authenticus ID: P-003-W0X

DOI: 10.1007/s11240-008-9418-5

Abstract (EN): The low induction rates of somatic embryogenesis (SE) in Eucalyptus globulus hamper scaling up the process for commercialization. We analyzed the effectiveness of several media (MS, 1/2MS, B5, WPM, DKW and JADS) during SE induction and expression. MS and B5 were the best media for SE induction and embling regeneration. In general, MS was the best medium for expression, independently of the medium previously used during induction. Several anti-browning compounds (ascorbic acid, charcoal, DTE, DTT, PVP, PVPP and silver nitrate) were added to the expression medium (MS), but all decreased SE potential and only DTE, charcoal and silver nitrate reduced explant browning. When added only during the induction period, anti-browning agents reduced accumulation of phenolics but also severely reduced SE potential. Continuous exposure completely inhibited the SE response. The negative impact of anti-browning agents on SE potential raises a question about the role of production/accumulation of phenolics in the SE process.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10