Somatic embryogenesis induction in leaves and petioles of a mature wild olive

• Type: Article in International Scientific Journal
• Date: 2010

Authors

Capelo, AM (Author) Other; Silva, S (Author) Other; Brito, G (Author) Other; Santos, C (Author) Other

Journal

Title: Plant Cell, Tissue and Organ Culture

Vol. 103

Pages: 237-242

ISSN: 0167-6857

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 31 Citations

Scopus - 32 Citations

Other information

Authenticus ID: P-003-1PN

DOI: 10.1007/s11240-010-9773-x

Abstract (EN): We describe a protocol for somatic embryogenesis (SE) induction from an adult wild olive tree (Olea europaea ssp. europaea var. sylvestris. The protocol used confirms for the first time that there is no need to use juvenile or rejuvenated material for SE induction. For SE induction, petiole and leaf (proximal, intermediary and distal zones) explants were grown on Murashige and Skoog (MS) or Olive Medium (OM) media with different combinations of plant growth regulators (PGR): alpha- naphthaleneacetic acid (NAA), Zeatin (Zea), indole-3-butyric acid (IBA), 2-isopentyl adenine (2iP), thidiazuron (TDZ) and 6-benzylaminopurine (BAP). All media had 30 g/l sucrose and 7 g/l agar, and the pH was adjusted to 5.8. Cultures were incubated in the dark and, after 3 months, they were transferred to MS medium without PGR for expression. Petiole explants gave the highest callus production, while for SE induction and expression distal blade leaf and petiole explants gave the highest rates. The best medium for SE induction was MS with 12.25 mu M IBA plus 4.56 mu M Zea. Histological analyses confirmed the individuality of globular somatic embryos. This is the first report of SE expression in explants without rejuvenation in Olea genus, and opens perspectives for using this strategy in SE protocols both for this wild genotype and for commercial genotypes.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6