Go to:
Logótipo
Você está em: Start > Publications > View > Preliminary evaluation of the in vitro cytotoxicity of PMMA-co-EHA bone cement
Map of Premises
Principal
Publication

Preliminary evaluation of the in vitro cytotoxicity of PMMA-co-EHA bone cement

Title
Preliminary evaluation of the in vitro cytotoxicity of PMMA-co-EHA bone cement
Type
Article in International Scientific Journal
Year
2011
Authors
Almeida, T
(Author)
Other
The person does not belong to the institution. The person does not belong to the institution. The person does not belong to the institution. Without AUTHENTICUS Without ORCID
Ferreira, BJML
(Author)
Other
The person does not belong to the institution. The person does not belong to the institution. The person does not belong to the institution. View Authenticus page Without ORCID
Loureiro, J
(Author)
Other
The person does not belong to the institution. The person does not belong to the institution. The person does not belong to the institution. View Authenticus page Without ORCID
Correia, RN
(Author)
Other
The person does not belong to the institution. The person does not belong to the institution. The person does not belong to the institution. Without AUTHENTICUS Without ORCID
Journal
Vol. 31
Pages: 658-662
ISSN: 0928-4931
Publisher: Elsevier
Other information
Authenticus ID: P-002-SF1
Abstract (EN): This work reports a preliminary in vitro cytotoxicity assessment of new poly (methyl methacrylate)-co-ethyl hexylacrylate (PMMA-co-EHA) bone cement by evaluating the effect of its leachables on the viability of human osteoblast-like cells (MG63 line) and their progression through the cell cycle. MG63 cells were exposed to 72 h-extract dilutions of PMMA-co-EHA and their viability was tested using the 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT) colorimetric assay. Also, putative changes in the progression of cells through the cell cycle were monitored using flow cytometry. For that the relative nuclear DNA content and the ratio of cells at G(1):S:G(2) stages of the cell cycle were measured after three exposure periods (24,48 and 72 h). The obtained results revealed a dose-dependent influence of the cement extract in MG63 cell metabolism when compared to cells cultivated in a culture medium only. The MTT assay showed that a moderate number of cells died after exposure to the most concentrated extract. The cell cycle analysis revealed that leachables of PMMA-co-EHA led to significant changes in cellular proliferation, with cells exposed for 48 h to the most concentrated extract being arrested in the S phase of the cell cycle. However, despite the initial period of cytotoxicity, the obtained results suggest that after 72 h of exposure, the surviving cells are able to recover from this arresting condition and continue to proliferate. Therefore, this preliminary study indicates that, at the biological level, PMMA-co-EHA may have potential of being used as a bone cement matrix. However, a more detailed research work is needed to fully understand the factors responsible for the initial cytotoxicity observed.
Language: English
Type (Professor's evaluation): Scientific
No. of pages: 5
Documents
We could not find any documents associated to the publication.
Related Publications

Of the same journal

3-D biomodelling technology for maxillofacial reconstruction (2008)
Article in International Scientific Journal
Manuel Oliveira; Sooraj S Hussain; Dias, AG; Lopes, MA; Luis Azevedo; Horacio Zenha; Horacio Costa; Santos, JD
Using plasma-mediated covalent functionalization of rhamnolipids on polydimethylsiloxane towards the antimicrobial improvement of catheter surfaces (2022)
Article in International Scientific Journal
Dardouri, M; Bettencourt, A; Victor Martin; Carvalho, FA; Santos, C; Monge, N; Santos, NC; Fernandes MH; Gomes PS; Ribeiro, IAC
Testing the variability of PSA expression by different human prostate cancer cell lines by means of a new potentiometric device employing molecularly antibody assembled on graphene surface (2016)
Article in International Scientific Journal
Rebelo TSCR; Noronha JP ; Galésio M ; Santos H; Diniz M ; Sales MGF; Fernandes MH; João Costa-Rodrigues
Sulphonamide-imprinted sol-gel materials as ionophores in potentiometric transduction (2011)
Article in International Scientific Journal
Almeida, SAA; Moreira, FTC; Heitor, AM; Montenegro, MCBSM; Aguilar, GG; Sales, MGF
Silk fibroin/nanohydroxyapatite hydrogels for promoted bioactivity and osteoblastic proliferation and differentiation of human bone marrow stromal cells (2018)
Article in International Scientific Journal
Marta Ribeiro ; Maria H. Fernandes ; Marisa M. Beppu; Fernando J. Monteiro; Ferraz, MP

See all (33)

Recommend this page Top
Copyright 1996-2025 © Faculdade de Medicina Dentária da Universidade do Porto  I Terms and Conditions  I Acessibility  I Index A-Z
Page created on: 2025-08-23 at 06:40:09 | Privacy Policy | Personal Data Protection Policy | Whistleblowing | Electronic Yellow Book